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Table 1 Comparison of recovery of Mycobacterium avium subsp. paratuberculosis by real-time PCR and culture

From: Enumeration of Mycobacterium avium subsp. paratuberculosis by quantitative real-time PCR, culture on solid media and optical densitometry

Isolate ODa F57qPCR Culture Differencef
   Copies/mlb SDc SCd CFU/mle SDc SCd  
8819 0.382 4.13 × 108 5.24 × 107 0.972 6.77 × 106 2.01 × 106 0.994 1.79
  0.197 1.71 × 108 5.43 × 106   3.98 × 106 1.10 × 106   1.63
  0.083 6.69 × 107 5.73 × 106   2.02 × 106 8.84 × 105   1.52
  0.032 3.22 × 107 3.27 × 106   9.58 × 105 3.23 × 105   1.53
  0.013 1.51 × 107 1.82 × 106   5.05 × 105 2.32 × 105   1.47
  0.004 7.35 × 106 4.78 × 105   2.03 × 105 8.59 × 104   1.56
  0.000 3.94 × 106 8.55 × 105   1.00 × 105 4.14 × 104   1.59
8672 0.402 7.09 × 108 1.84 × 107 0.998 5.42 × 106 1.85 × 106 0.990 2.12
  0.184 2.70 × 108 2.30 × 107   2.77 × 106 9.99 × 105   1.99
  0.082 1.11 × 108 5.42 × 106   1.22 × 106 3.01 × 105   1.96
  0.045 5.42 × 107 4.58 × 106   5.10 × 105 1.04 × 105   2.03
  0.019 2.44 × 107 1.25 × 106   2.50 × 105 8.75 × 104   1.99
  0.008 1.07 × 107 9.01 × 105   1.28 × 105 6.84 × 104   1.92
  0.000 5.48 × 106 5.29 × 105   5.45 × 104 2.44 × 104   2.00
12146 0.281 3.89 × 108 5.45 × 107 0.989 1.83 × 106 6.25 × 105 0.968 2.33
  0.135 1.51 × 108 1.86 × 107   1.09 × 106 6.15 × 105   2.14
  0.052 6.80 × 107 4.95 × 106   6.17 × 105 3.76 × 105   2.04
  0.030 2.99 × 107 2.02 × 106   2.52 × 105 1.17 × 105   2.07
  0.012 1.48 × 107 1.64 × 106   1.38 × 105 9.16 × 104   2.03
  0.006 7.37 × 106 1.20 × 106   3.22 × 104 1.20 × 104   2.36
  0.000 3.26 × 106 2.87 × 105   2.48 × 104 1.10 × 104   2.12
  1. qPCR: quantitative real-time PCR
  2. a OD: optical density at 600 nm, values represent mean of three independent measurements
  3. b Mean number of F57 copies (which is equal to number of Mycobacterium avium subsp. paratuberculosis cells) from a triplicate in a respective dilution
  4. c Standard deviation
  5. d Square Spearman's rank correlation coefficient (R2) expressing linear dependence of F57qPCR (or culture) counts with OD
  6. e Mean number of CFU recovered by culture on solid media from triplicate in a respective dilution
  7. f Difference in log10 between mean value gained by F57qPCR and culture expressed as log10(copies/ml) - log10(CFU/ml)