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Table 1 Comparison of recovery of Mycobacterium avium subsp. paratuberculosis by real-time PCR and culture

From: Enumeration of Mycobacterium avium subsp. paratuberculosis by quantitative real-time PCR, culture on solid media and optical densitometry

Isolate

ODa

F57qPCR

Culture

Differencef

  

Copies/mlb

SDc

SCd

CFU/mle

SDc

SCd

 

8819

0.382

4.13 × 108

5.24 × 107

0.972

6.77 × 106

2.01 × 106

0.994

1.79

 

0.197

1.71 × 108

5.43 × 106

 

3.98 × 106

1.10 × 106

 

1.63

 

0.083

6.69 × 107

5.73 × 106

 

2.02 × 106

8.84 × 105

 

1.52

 

0.032

3.22 × 107

3.27 × 106

 

9.58 × 105

3.23 × 105

 

1.53

 

0.013

1.51 × 107

1.82 × 106

 

5.05 × 105

2.32 × 105

 

1.47

 

0.004

7.35 × 106

4.78 × 105

 

2.03 × 105

8.59 × 104

 

1.56

 

0.000

3.94 × 106

8.55 × 105

 

1.00 × 105

4.14 × 104

 

1.59

8672

0.402

7.09 × 108

1.84 × 107

0.998

5.42 × 106

1.85 × 106

0.990

2.12

 

0.184

2.70 × 108

2.30 × 107

 

2.77 × 106

9.99 × 105

 

1.99

 

0.082

1.11 × 108

5.42 × 106

 

1.22 × 106

3.01 × 105

 

1.96

 

0.045

5.42 × 107

4.58 × 106

 

5.10 × 105

1.04 × 105

 

2.03

 

0.019

2.44 × 107

1.25 × 106

 

2.50 × 105

8.75 × 104

 

1.99

 

0.008

1.07 × 107

9.01 × 105

 

1.28 × 105

6.84 × 104

 

1.92

 

0.000

5.48 × 106

5.29 × 105

 

5.45 × 104

2.44 × 104

 

2.00

12146

0.281

3.89 × 108

5.45 × 107

0.989

1.83 × 106

6.25 × 105

0.968

2.33

 

0.135

1.51 × 108

1.86 × 107

 

1.09 × 106

6.15 × 105

 

2.14

 

0.052

6.80 × 107

4.95 × 106

 

6.17 × 105

3.76 × 105

 

2.04

 

0.030

2.99 × 107

2.02 × 106

 

2.52 × 105

1.17 × 105

 

2.07

 

0.012

1.48 × 107

1.64 × 106

 

1.38 × 105

9.16 × 104

 

2.03

 

0.006

7.37 × 106

1.20 × 106

 

3.22 × 104

1.20 × 104

 

2.36

 

0.000

3.26 × 106

2.87 × 105

 

2.48 × 104

1.10 × 104

 

2.12

  1. qPCR: quantitative real-time PCR
  2. a OD: optical density at 600 nm, values represent mean of three independent measurements
  3. b Mean number of F57 copies (which is equal to number of Mycobacterium avium subsp. paratuberculosis cells) from a triplicate in a respective dilution
  4. c Standard deviation
  5. d Square Spearman's rank correlation coefficient (R2) expressing linear dependence of F57qPCR (or culture) counts with OD
  6. e Mean number of CFU recovered by culture on solid media from triplicate in a respective dilution
  7. f Difference in log10 between mean value gained by F57qPCR and culture expressed as log10(copies/ml) - log10(CFU/ml)
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BMC Research Notes

ISSN: 1756-0500

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