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Table 1 Nuclear protein extract concentration and quality estimated by the contamination by non nuclear (cytosolic) fraction nd: not detected; the same letter indicates that values are not significantly different (P > 0.05). Value is mean ± standard error

From: Isolation of nuclear proteins from flax (Linum usitatissimum L.) seed coats for gene expression regulation studies

  Commercial kit Published method 1[10] Published method 2[11] Optimized protocol (present study)
Protein concentration (μg.mg -1 FW) 168.5 ± 7.2a 188.2 ± 6.6b 173.8 ± 9.0b 247.8 ± 6.1 c
AdH specific activity (AU.mg -1 FW) 20.7 ± 3.0c 8.8 ± 1.2bc 15.9 ± 3.8c nd a