Figure 2

Analysis of the modified BAC by junction PCRs. Schematic representation of the performed junction PCRs is depicted. The CreERT²-Neo targeted region of the BAC with the corresponding primer binding sites (arrows) and the expected sizes of the PCR products are indicated. Homologous regions are depicted in green. Agarose gel electrophoresis demonstrated that all four junction PCRs yielded products of the correct size.