An investigation of horizontal transfer of feed introduced DNA to the aerobic microbiota of the gastrointestinal tract of rats

Table 4 PCR primers used in this study

Target	Name	Size	Primer sequence (5'-3' direction)	References
Insertion of pM2	KanR-F	800 bp	ATC GCA GTG GTG AGT AAC	This study,
	ITSR-Eub		GCC AAG GCA TCC ACC	[51]
	16S-926 F	850 bp	AAA CTT AAA TGA ATT GAC GC	[52]
	GFP-R		TTC TTT TGT TTG TCT GCC ATG ATG TAT	This study
Insertion of pM3	16S-PrMir530F	1030 bp	GTG CCA GCA GC GCG G	This study
	GFP-F		ATA CAT CAT GGC AGA CAA ACA AAA GAA	This study
16S	10 F	900 bp	AGT TTG ATC ATG GCT CAG ATT G	[53, 54]
	907R		CCG TCA ATT CHT TTR AGT TT
16S of A. baylyi	F16	1000 bp	ACT AGC GGA TCC GAC TTC	This study
	F17		CCA GAC TTC TAG AGG AGG C
23S of Psychrobacter	F13	1258 bp	CGT TGG ACT CGA GCC CTT G	This study
	F14		CACATGGTGGATCCCTTGG
16S gfp	E9F-K1	1500 bp	GAG TTT GAT CCT GGA TCA	This study
			TTG GCC ATG GAA CAG GTA
km^R 23S	Kmup-97ar	2748 bp	GTA TGA GTC AGC AAC ACC TTC	This study
			CGC TTA GAT GCT TTC AGC

ISSN: 1756-0500