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Table 4 PCR primers used in this study

From: An investigation of horizontal transfer of feed introduced DNA to the aerobic microbiota of the gastrointestinal tract of rats

Target

Name

Size

Primer sequence (5'-3' direction)

References

Insertion of pM2

KanR-F

800 bp

ATC GCA GTG GTG AGT AAC

This study,

 

ITSR-Eub

 

GCC AAG GCA TCC ACC

[51]

 

16S-926 F

850 bp

AAA CTT AAA TGA ATT GAC GC

[52]

 

GFP-R

 

TTC TTT TGT TTG TCT GCC ATG ATG TAT

This study

Insertion of pM3

16S-PrMir530F

1030 bp

GTG CCA GCA GC GCG G

This study

 

GFP-F

 

ATA CAT CAT GGC AGA CAA ACA AAA GAA

This study

16S

10 F

900 bp

AGT TTG ATC ATG GCT CAG ATT G

[53, 54]

 

907R

 

CCG TCA ATT CHT TTR AGT TT

 

16S of A. baylyi

F16

1000 bp

ACT AGC GGA TCC GAC TTC

This study

 

F17

 

CCA GAC TTC TAG AGG AGG C

 

23S of Psychrobacter

F13

1258 bp

CGT TGG ACT CGA GCC CTT G

This study

 

F14

 

CACATGGTGGATCCCTTGG

 

16S gfp

E9F-K1

1500 bp

GAG TTT GAT CCT GGA TCA

This study

   

TTG GCC ATG GAA CAG GTA

 

kmR 23S

Kmup-97ar

2748 bp

GTA TGA GTC AGC AAC ACC TTC

This study

   

CGC TTA GAT GCT TTC AGC