Table 1 Nucleotide base sequences of primers

For amplification of cDNA

1

5'-GCGGCACCTCGGAAGGC-3'

5'-CCCCAAACTTTGACTTTTAGC-3'

60°C

1 min

Figure 1 A 1

834 bp

2

5'-GATCGGTTTATCCCTTGTGGTC-3'

5'-CTTCAGGTTCTTTAAAGTTTGG-3'

60°C

1 min

Figure 1 A 2

865 bp

3

5'-CTGAAGGGATGTCCAATGC-3'

5'-ATATTTTATATGATTCTTTTCCTC-3'

56.1°C

1 min

Figure 1 A 3

850 bp

4

5'-CCAGTCTGTTAACTCCTAGC-3'

5'-GGATAATGTTCCTCAATATCTTTG-3'

60°C

1 min

Figure 1 A 4

826 bp

5

5'-ACAGCTTCTAATAAAGAGATAAA AC-3'

5'-GCCGGCATTTATTATTTTTC-3'

56.1°C

1 min

Fig. 1 A 5

850 bp

6

5'-GTTTCTCCTCAAGCAAATACAA-3'

5'-ATTTTTTACTTTGTCCAAAGATTCC-3'

60°C

1 min

Figure 1 A 6

873 bp

7

5'-CTGATCCTGCAGCAAAGACC-3'

5'-GAAAAACCAATGTTTTTTCTCTCTC-3'

59.2°C

1 min

Figure 1A 7

908 bp

8

5'-CATTCTAGTGAAGTGTATAATAA ATCAG-3'

5'-CTGTCCTAAATCCAGAGAAAGC-3'

50.8°C

1 min

Figure 1 A 8

919 bp

9

5'-AGTATCACTTAAAGATAATGAAG AAC-3'

5'-CTTTTAGGATGCCGTCTGG-3'

50°C

1 min

Figure 1 A 9

887 bp

10

5'-CCCCCAATTAAAAGAAACTTG-3'

5'-GCCAATTGTATTCCTTCTCC-3'

53.7°C

1 min

Figure 1 A 10

905 bp

11

5'-CCTCTGCATGTTCTCATAAAC-3'

5'-GGGTATGCTCTTTGAACAACTAC-3'

60°C

1 min

Figure 1 A 11

886 bp

12

5'-CATGGAGCAGAACTGGTAGG-3'

5'-GTGTAAGGTTTAATAATGTCTTCA-3'

50°C

1 min

Figure 1 A 12

1094 bp

13

5'-CCTATCCCAAGTTTATCAGCC-3'

5'-CAGACACAAGTTGATGTTCTCC-3'

60°C

1 min

Figure 1 A 13

959 bp

14

5'-GAAGGCATTTCAGCCACCACG-3'

5'-CAATCACACTAGAATCATAAAAAGG-3'

60°C

1 min

Figure 1 A 14

978 bp

For amplification of genomic DNA

exon 1-2

5'-GCCCCCTGCCCAGAACCC-3'

5'-CTTTTCAGCAAGCATGCACAGTTACG-3'

60°C

2 min

Figure 1 B 1

1193 bp

exon 3

5'-CTACAGTCAAAATGTCAAGCG-3'

5'-CACAATTAACAATAGATCTGGGAG-3'

60°C

1 min

Figure 1 B 2

430 bp

exon 4-7

5'-ATAAGAATAAAAACTTCCAGAGAATG-3'

5'-ATTATCTTTTCATATATTCTTTTTGTC-3'

60°C

2 min

Figure 1 B 3

1384 bp

exon 8-9

5'-GTAGTATATGTGACTTTTGATGTCTG-3'

5'-GGAAAAGCAATGTATTTTCTCTTT-3'

60°C

2 min

Figure 1 B 4

615 bp

exon 10

5'-CTTTAAATACTGCCTTATGGGCTA-3'

5'-GTCACCATCCCTAAAACTATATGC-3'

60°C

2 min

Figure 1 B 5

1311 bp

exon 11-a

5'-GTCACTTTGTGTCTTCATGC-3'

5'-GGATAATGTTCCTCAATATCTTTG-3'

56.4°C

2 min

Figure 1 B 6

1246 bp

exon 11-b(same as primer set 5)

5'-ACAGCTTCTAATAAAGAGATAAAAC-3'

5'-GCCGGCATTTATTATTTTTC-3'

56.4°C

1 min

Figure 1 B 7

850 bp

exon 11-c(same as primer set 6)

5'-GTTTCTCCTCAAGCAAATACAA-3'

5'-GATTTTTTACTTTGTCCAAAGATTCC-3'

60°C

1 min

Figure 1 B 8

873 bp

exon 11-d(same as primer set 7)

5'-CTGATCCTGCAGCAAAGACC-3'

5'-GAAAAACCAATGTTTTTTCTCTCTC-3'

59.2°C

1 min

Figure 1 B 9

908 bp

exon 11-e

5'-CATTCTAGTGAAGTGTATAATAAATCAG-3'

5'-ATTCCCCTAAACTATACATAAAAG-3'

56.4°C

2 min

Figure 1 B 10

1720 bp

exon 12

5'-CAATTCTTTAGTTTTAAAAAATGG GC-3

5'- GAAAAAGCTTAGAAAAAGAACTTAAAAAATAC-3'

59.2°C

1 min

Figure 1 B 11

275 bp

exon 13

5'- GTAAATGTTTATAATGTGTAATATACAGGC-3'

5'-CTGTACCTTCCCTACACTATATTAGTAG-3'

60°C

1 min

Figure 1 B 12

230 bp

exon 14-15

5'-CCAAACTTAAATATTTTCTCCTC-3'

5'-CAGGGATCCCAGTCTATTC-3'

60°C

2 min

Figure 1 B 13

1213 bp

exon 16

5'-GCAGCAAACCCTTGAATGTAG-3'

5'-GTCAGGTGAACCGTAATGTG-3'

60°C

1 min

Figure 1 B 14

552 bp

exon 17-8

5'- GGTCTTGTACAGTGTAGTGTTTG-3'

5'-GTTTTTAAGCAATGGAGCATC-3'

59.2°C

2 min

Figure 1 B 15

1258 bp

exon 19-20

5'- CCATCATGTTTAAATTGAAGTCTC-3'

5'-CAATTACAGAGGTTAAATCAGAAGCC-3'

59.2°C

2 min

Figure 1 B 16

739 bp

exon 21-24

5'-CTCGATATTTGATTCACCAGC-3'

5'-CAACAGTCCCTTCCTAACCC-3'

60°C

2 min

Figure 1 B 17

1739 bp

exon 25

5'- CAGTATCACTTTTTCTACATTTTG GTC-3'

5'-CCCAATTTTCACAGAAGCCAC-3'

59.2°C

1 min

Figure 1 B 18

471 bp

exon 26

5'-GGCTTCCATAGATGTTAGATG-3'

5'-GGACAACTTGGGATCATTTGC-3'

50.8°C

1 min

Figure 1 B 19

337 bp

exon 27

5'- GCTAAATTGCTGATGTTTTCTAC-3'

5'-CTGCTGAGTCCTCTAATAAGGC-3'

60°C

2 min

Figure 1 B 20

1437 bp

exon 25

5'- CAGTATCACTTTTTCTACATTTTGGTC-3'

5'-CCCAATTTTCACAGAAGCCAC-3'

59.2°C

1 min

Figure 1 B 18

471 bp

exon 26

5'-GGCTTCCATAGATGTTAGATG-3'

5'-GGACAACTTGGGATCATTTGC-3'

50.8°C

1 min

Figure 1 B 19

337 bp

exon 27

5'- GCTAAATTGCTGATGTTTTCTAC-3'

5'-CTGCTGAGTCCTCTAATAAGGC-3'

60°C

2 min

Figure 1 B 20

1437 bp

For detection of splicing variants

exon 1-11

5'-CGAATTTGTTAGCCGTCTCC-3'

5'-GGATCCTGAGATATTATTTTATTATTAG-3'

60°C

2.5 min

Figure 1 C 1

2118 bp

exon 10-14

5'-CTGAAGGGATGTCCAATGC-3'

5'-GAAATTTGGATTCTGTATTTCTTG-3'

58°C

6 min

Figure 1 C 2

5594 bp and 1554 bp

exon 11-18

5'-CTTCCTGTGAAAACAAATATAG-3'

5'-GCTGATCTTCTGCTTTTATC-3'

50.8°C

2 min

Figure 1 C 3

1417 bp

exon 15-25

5'-CCTCTGCATGTTCTCATAAAC-3'

5'-GTGTAAGGTTTAATAATGTCTTCA-3'

60°C

2 min

Figure 1 C 4

1759 bp

exon 24-27 (same as primer set 13)

5'-CCTATCCCAAGTTTATCAGCC-3'

5'-CAGACACAAGTTGATGTTCTCC-3'

60°C

2 min

Figure 1 C 5

959 bp

For nested PCR of the transcripts lacking most of exon 11

exon 10-13(1735-7280)

5'-GTTCTCAAATAATATGACTAATCCAAAC-3'

5'-GTTCCTCAGTTGTGCGAAAG-3'

58°C

6 min

Figure 2 A

5546 bp and 1506 bp, 1185 bp, 1674 bp or 1270 bp

For DNA sequence

cB2 seq1

5'-CAATAGAGGTGTTTTCTCCATC-3'

cB2 seq2

5'-GGATCCTGAGATATTATTTTATTATTAG-3'

5546 bp and 1506 bp, 1185 bp, 1674 bp or 1270 bp

cB2 seq3

5'-CCAGCTTTGTCTTTAACCAG-3'

cB2 seq4

5'-CTGTGTGACCACTTTCACTATC-3'

cB2 seq5

5'-CCCTCCTTCATAAACTGGC-3'

cB2 seq6

5'-CTTTCTGAGAGGCATGATCTG-3'

cB2 seq7

5'-GCATGGCAAGTGTCTGATTTAC-3'

cB2 seq8

5'-GTGAACAAACTTCACAACTTAACC-3'

cB2 seq9

5'-GCTGATCTTCTGCTTTTATC-3'

cB2 seq10

5'-GGTATGTTTTACAATGATGC-3'

cB2 ex14 R (exon)

5'-CTAAAGGTTCTTTTTCATTCTTTG-3'

cB2 ex15 F

5'- GCTTTTTAAATGTTACATGGAGG-3'

cB2 ex17 R

5'-GTACCAGTCAGGGATGTGAG-3'

cB2 ex18 F (exon)

5'-ATATGATGTGGAAATTGATAAAA G-3'

cB2 ex22F

5'-CTTTTTAAAGGGATTCATTTACAG TGG-3'

cB2 ex23 F (exon)

5'-CCATCACCAGATTTATATTCCC-3'

cB2 ex26 R (exon)

5'-CAGAAATTTATTTCCTATGCC-3'

cB2 ex23 F (exon)

5'-CCATCACCAGATTTATATTCCC-3'

cB2 ex26 R (exon)

5'-CAGAAATTTATTTCCTATGCC-3'