Figure 4
Fluorescence microscopy of retinal progenitor marker expression in AF-treated (Left) and FBS-treated (Right) RPE cells. A, E: Nuclear localization of CHX10 in cells (A: AF-treated cells, E: FBS-treated cells) immunostained with goat polyclonal anti-human CHX10 antibody. B, F: Nuclear localization of PAX6 in cells (B: AF-treated cells, F: FBS-treated cells) immunostained with goat polyclonal anti-human PAX6. C, D, G, H: The nuclei of each field were counter-stained with DAPI. Note that AF-treated RPE cultures have an approximately 3-fold increase in retinal progenitor marker expression (33% and 27% for CHX10 and PAX6, respectively) when compared with that of FBS-treated cultures. Each value represents the mean of 3 independent experiments in at least triplicate. Magnifications; A &C: 200, B, D, E, F, G &H: 400.