Figure 5

Analysis of RPE cell proliferation (Left) and death (Right). An ELISA assay was carried out as described in the methods. Left: Comparison of RPE cell proliferation between AF-treated cultures (10%, 20%, 30%), FBS-treated cultures (10%) and DMEM/F12 (control)-treated cultures. Note the dose-dependent increase in AF-treated RPE cell proliferation. Right: Comparison of RPE cell death in AF-treated cultures (10%, 20%, 30%), FBS-treated cultures (10%) and DMEM/F12 (control)-treated cultures with positive control (DNA-histone complex). Note that AF does not have any apoptotic effects on RPE cells at any of the concentrations tested. Comparative analysis of AF and FBS-treated cultures indicates that AF may be a suitable substrate for FBS replacement in culture medium. Each value represents the meanSEM of 4 independent experiments in at least triplicate.