Insert size distribution in a random sample of 192 BAC clones of the SP80-3280 sugarcane BAC library. (A) Example of pulsed-field gel electrophoresis (PGE) of 48 BAC clones DNA digested with Not I. Lanes 1, 26 and 51 are Lambda Ladder PFG (New England Biolabs) molecular weight DNA markers. The 7.5-Kb band marks the position of the Not I-released cloning vector. (B) Insert size distribution of the 192 BAC clones as estimated by Not I digestion and PGE.