A BAC library of the SP80-3280 sugarcane variety (saccharum sp.) and its inferred microsynteny with the sorghum genome

Table 2 Classification of SP 80–3280 BAC end sequences as related to the alignments into the sorghum chromosomes

BES Class	BES per BAC	Aligned BES	Sorghum Chromosome	BES Orientation	Distance Between BES (Kb)	Type	BAC Count
1	2	2	Same	Opposite in (> <)	20 - 300	Concordant	42
2	2	2	Same	Same (< < or > >)	20 - 300	Discordant	1
3	2	2	Same	Opposite in (> <)	> 300	Discordant	16
4	2	2	Same	Same (<< or >>)	> 300	Discordant	12
5	2	2	Same	Opposite out (< >)	> 300	Discordant	15
6	2	2	Different	N/A	N/A	Discordant	9
7	2	1	N/A	N/A	N/A	1	10
8	2	1	N/A	N/A	N/A	> 1	65
9	2	0	N/A	N/A	N/A	N/A	22

Class 1, BAC end pairs that matched the same Sorghum chromosome at positions 20 to 300 Kb apart in opposite orientation. Class 2, BAC end pairs that matched the same Sorghum chromosome at positions 20 to 300 Kb apart in the same orientation. Class 3, BAC end pairs that matched the same Sorghum chromosome within a distance larger than 300Kb in the opposite in orientation. Class 4, BAC end pairs that matched the same Sorghum chromosome within a distance larger than 300Kb in the same orientation. Class 5, BAC end pairs that matched the same Sorghum chromosome within a distance larger than 300Kb in the opposite out orientation. Class 6, BAC end pairs that matched different Sorghum chromosome. Class 7, BAC end pairs for which only one sequence matched a sorghum chromosome at a single position. Class 8, BAC end pairs for which only one sequence matched sorghum chromosomes in more than one position. Class 9, BAC end pairs that didn’t match Sorghum chromosome.

ISSN: 1756-0500