Figure 4
Effects of HPLC samples of the fractionated SRL hot-water extract on MTS-C H7 cell proliferation. (A) Major catechins, i.e., (−)-epicatechin (EC), (−)-epicatechin gallate (ECG), (−)-epigallocatechin (EGC), and (−)-epigallocatechin gallate (EGCG), in green tea extract and SRL extract. Fraction 1 (F1): 100% solvent A, 0–10.0 min; fraction 2 (F2): 90% solvent A + 10% solvent B, 10.1–40.0 min; and fraction 3 (F3): 40.1–80.0 min. (B) Anti-proliferative effects of the fractionated samples of the SRL extract on the growth of MTS-C H7 cells after 24-h treatment. MTS-C H7 cells were treated for 24 h with mouse IL-1β (10 ng/mL); SRL extract (25 μg dry matter/mL); or F1, F2, and F3 (25 μg dry matter weight/mL). Cell proliferation was assessed by the WST-1 assay. The results are expressed as mean (SD), n = 3. Significantly different from IL-1β: * p < 0.05. The experiment was performed in triplicate.