Figure 6

Characterization of the active ingredients as peptides. SRL extract (0.5 mg dry matter) was mixed with carboxypeptidase Y (CPaseY) (2.38 units) and incubated at 25°C for 24 h. After incubation, the samples were heated at 95°C for 5 min. SRL extract was mixed with HCl (6 mol/L) and heated at 90°C for 2 h. (A) The SRL-only sample was subjected to electrophoresis (lane 2). SRL was treated with CPaseY (lane 3). SRL was treated HCl (lane 4). Proteins were detected by SDS-PAGE with gels stained with GelCode Blue Safe Protein Stain. (B) MTS-C H7 cells were treated with mouse IL-1β (10 ng/mL) and SRL treated with CPaseY. Cell proliferation was assessed by the WST-1 assay. The results are expressed as mean (SD), n = 3. Significantly different from IL-1β: * p < 0.05. The experiment was performed in triplicate.