Figure 1From: Akt and phospholipase Cγ are involved in the regulation of growth and migration of MDA-MB-468 breast cancer and SW480 colon cancer cells when cultured with diabetogenic levels of glucose and insulinWestern blot analysis of Akt and PLCγ expression. Western blot analysis was performed to evaluate Akt and PLCγ expression and shows that Akt expression was up-regulated in both cell lines when additional glucose was available (A). The combination of glucose and insulin caused an even higher up-regulation in both cell lines (A). PLCγ expression showed considerable increase in SW480 colon cancer cells exclusively (B). Numbers indicate relative Akt and PLCγ expression levels in relation to β-actin, whereby expression levels in the 5.5 mM glucose control media were set to 100%.Back to article page