Figure 1
From: Hepatitis B surface gene 145 mutant as a minor population in hepatitis B virus carriers
Mutant-specific real-time PCR was performed in duplicate for each probe. (A) HBV DNA from serum with an escape mutant (single mutation at nt 587, A to G) was amplified using the wild-type and mutant 1, 2, and 3 probes. (B) Constructed plasmid mutant-type (single mutation at nt 587 G to A) DNA and wild-type DNA were mixed. The amount of the mutant-type plasmid DNA was equal to that of the wild-type DNA. The curves of the amplification plots are indicated by arrows. (C) Constructed plasmid mutant-type (single mutation at nt 587 G to A) DNA and wild-type DNA were mixed. The mutant:wild ratio in plasmid DNA was 1:10. (D) Constructed plasmid mutant-type (single mutation at nt 587 G to A) DNA and wild-type DNA were mixed. The mutant:wild ratio in plasmid DNA was 1:100.