SDS-PAGE of the purified recombinant proteins. (A) HhXyl and (B) XIP-I from wheat. The proteins were purified by affinity chromatography using Ni-NTA resin. Lane 1: Low range weight marker (Bio-Rad), Lane 2: negative control for expression, Lane 3: Unpurified samples, Lane 4: 5 μl of the concentrated pure HhXyl (A) and XIP-I (B) proteins. The proteins were visualized with Coomassie brilliant blue R250.