Figure 3

Coverage of the 18S rRNA and actin genes. The coverage of reads across the 18S rRNA (Panel A) and actin (Panel C) genes was determined by mpileup in samtools and compared between total RNA (solid line) and Ribo-Zero-subtracted RNA (dashed line). For the 18S rRNA, the Ribo-Zero-subtracted sample contained 66% fewer rRNA reads when compared to the total RNA sample. When the 18S rRNA results are normalized relative to actin, a 95% reduction in the rRNA is seen (Panel B). The 18S rRNA is only partially sequenced in the reference genome with a gap in the scaffold to the immediate right of this region.