Table 3 Shows the sequences of the amplification primers used for PCR
x | PCR primers (5'-3') | ||
|---|---|---|---|
Locus | Forward primer | Reverse primer | Amplicon Size (bp) |
Fcer1g | 5'-TTAGTGAGAGTCGAGGATCA-3' | 5'-TTCCATCTCTGTAGAAAGCA-3' | 480 |
Lipa | 5'-CTCCACAGAATGTTTCTCCA-3' | 5'-CCTAGTTCTCCAGAGGTTGA-3' | 501 |
Lrrc57 | 5'-GTGGCTGTTGTGTTGGTGCT-3' | 5'-TTGAGCCGTGTAGGTCATGG-3' | 342 |
Gramd4 | 5'-CTTCTAGGCATCCTTACAGA-3' | 5'-TAGACAGTTTTGTGCTCGAC-3' | 446 |
Snx5 | 5'-ATGATAGTCTTCCAACTTCTC-3' | 5'-TGATAGGTATTTGGATGCTG-3' | 384 |
Zfp235 | 5'-GTTGACTGAAGCATCACTTC-3' | 5'-TTCACCTTATTGGGTTGATA-3' | 383 |
Sspn | 5'-GTAGTAGAGTCTTCATTAAAGCC-3' | 5'-TCAGTGGCTTACACACTAAG-3' | 360 |