Figure 5

Effect of BSA as a co-additive with organic solvents in various PCR applications. A) A representative example of the effect of BSA addition (BSA Step) as a co-enhancer of DMSO effect on PCR amplification of cheA4 in a “Touchdown” PCR protocol, as detected by gel electrophoresis. B) A representative example of the effect of BSA Step protocol used in site-directed mutagenesis (QuickChange site-directed mutagenesis, Stratagene) PCR amplification of pUCtlp2, as detected by gel Electrophoresis. C) A representative example of the effect of BSA Step protocol used in an overlap extension PCR application, fusing ATM with CFP to yield ATM-CFP, as detected by gel Electrophoresis.