Table 1 Target DNA fragments and primers used in this study
Target DNA Fragment | Primer Name | Primer Sequence |
|---|---|---|
che1P | pFUS2Che1p Xho-F | 5′ CCCCTCGAGCGCGATGAACTGGTTG |
pFUS2Che1p Eco-R | 5′CCCGAATTCATCGGGTTTCATGGGAC | |
tlp5P | Tlp5FUSfor | 5′ CCAAGCTTCTGTCCGGCACCGTCTTC |
Tlp5FUSrev | 5′ CCCCTGGAGAGGCAGCAGGGTTTCG | |
tlp2 | Tlp2-Hind-F2 | 5′GGAAGCTTGTCACTCGGGCGACTCG |
Tlp2-Xho-R2 | 5′GGCCTCGAGTCACGCCGAAGGCGGG | |
cheA1 | gwCheA1-For2 | 5′GGGGACAAGTTTGTACAAAAAAGCAG |
CheA1GW-Rev | 5′ GGGGACCACTTTGTACAAGAAAGCTG | |
cheop1 | CheA1TMup | 5′ AACATGTCCCTGCTCAAGCAGCGTTCC |
CheR1dwn | 5′ AACATGTCCCTGCTCAAGCAGCGTTCC | |
ATM-CFP | ATM_CFP OL For | 5′ GACGTGGAACTGGTGAGCAAGGGC |
ATM_CFP OL REV | 5′ CTCGAGTTAAGATCTGTACAG | |
Ovl-ATM For | 5′ CCGCTCGAGCAGCGCGATGAACTGGTT | |
Ovl-ATM Rev | 5′ GTGGAACTGGTGAGCAAGGGC | |
pUCtlp2 | Tlp2-SDM3-For2 | 5′ GCGCAAACGACGCCAACGCCAGCA |
Tlp2-SDM3-Rev2 | 5′ GCGCCTGGCGGTTGCTGGCGTTGG | |
cheA4 | CheA4 H47Q-REV | 5′ CTTGATGGACTGGACGGCGCG |
CheA4 H47Q-FOR | 5′ CGCGCCGTCCAGTCCATCAAG |