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Figure 2 | BMC Research Notes

Figure 2

From: Ribozyme-mediated gene knock down strategy to dissect the consequences of PDGF stimulation in vascular smooth muscle cells

Figure 2

PDGFR-β knock-down assessment and toxicity. A) Histogram of Real-time PCR amplification of RNA recovered from VSMCs. Control bar represents the results obtained with VSMCs cultured in complete medium and normalized to 1 as reference value. Gray bars represent mean ± SE of three independent experiments. Statistical significance was calculated applying the unpaired t-test (* P < 0.05). B) Graph of summarizing MTT assay. VSMCs were cultured in standard condition for the control or in the presence of active (aHHR) and inactive ribozymes (iHHR). Each point represents the mean ± SD of 3 independent replicates

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BMC Research Notes

ISSN: 1756-0500

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