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Table 2 Influence of the co-cultivation media on transient expression efficiency of the rough lemon plants

From: Agrobacterium-mediated transformation of rough lemon (Citrus jambhiri Lush) with yeast HAL2 gene

Types co-cultivation media

Average (Percentage ± SE) of segments producing shoots

Standard deviation

Plain MS medium

5 (5.55 ± 0.3333)

0.5774

MS medium + 100 μM acetoseryngone

23 (25.55 ± 0.6667)

1.1574

MS medium + 200 μM acetoseryngone

40 (44.44 ± 0.6667)

1.1174

MS medium + 200 μM acetoseryngone

+ 0.2 mg L-1 2,4-D

24 (26.66 ±0.5774)

1.000

  1. Note. Data represent the mean value of three independent experiments. 75–100 explants were used in each experiment.
  2. All media contains MS salts and vitamins.