Diagnosis of HEV infection by serological and real-time PCR assays: a study on acute non-A-C hepatitis collected from 2004 to 2010 in Italy

Table 1 Positive results in serological and real-time PCR assays are shown in bold

Patient code	Results of serological assays^a		Results of real-time PCR (HEV-RNA^b)	HEV genotype	Country of origin *	ALT (UI/L)
Patient code	Bioelisa HEV - IgM	Bioelisa HEV - IgG	Results of real-time PCR (HEV-RNA^b)	HEV genotype	Country of origin *	ALT (UI/L)
8	>3.000	>3.000	+ + +	1a	Italy (India)	2640
3	1.304	>3.000	+ + +	1a	Bangladesh	1726
12	>3.000	>3.000	+ + +		India	1072
39	0.882	>3.000	+ + +		India	2463
16	1.801	>3.000	+ +	1a	Bangladesh	1263
19	1.695	>3.000	+ +		Bangladesh	835
22	>3.000	>3.000	+ +	1a	Italy (India)	n.a.
43	0.508	>3.000	+ +		Bangladesh	4515
47	0.867	>3.000	+ +		Bangladesh	1683
48	2.709	>3.000	+ +		Bangladesh	2050
52	0.869	>3.000	+ +	1a	Bangladesh	2496
55	1.316	>3.000	+ +		India	1263
56	1.508	>3.000	+ +		Bangladesh	n.a.
58	0.793	2.070	+ +		Italy (Thailand)	4044
60	2.665	0.775	+ +	3e	Italy	2010
7	0.498	>3.000	+ +		India	486
51	>3.000	>3.000	+ +		Italy	n.a.
61	0.630	1.972	+ +	1a	Italy (India)	2472
10	2.388	>3.000	+		Ethiopia	5328
29	2.212	>3.000	+		Bangladesh	n.a.
34	>3.000	2.969	+		Italy	855
63	0.102	0.088	+ + +		Albania	597
33°	0.057	1.354	+		Italy	384
20°	0.653	0.151	-		Italy (Africa)	over
30°	2.986	0.336	-		Pakistan	n.a.
50°	0.480	0.081	-		Italy	3860
67°	0.546	0.000	-		Italy (Africa)	842
26°	0.271	0.814	-		n.a.	2050
28°	0.062	0.709	-		Bangladesh	806
32°	0.126	1.226	-		Italy	2391

^a Bioelisa HEV IgM, (cut-off = 0.421); Bioelisa HEV IgG, (cut-off = 0.574); Biokit.
^b +: 250 < copies/mL < 2,500; + +: 2,500 < copies/mL <25,000; + + +: >25,000 copies/mL; -: < 250 copies/mL.
^* All foreign patients either arrived in Italy or travelled to their country of origin less than one month before the onset of clinical symptoms. Travel to countries from endemic areas are indicated in brackets for Italian patients.
n.a. : not available.
°Serum was also analysed with HEV IgM Elisa MP Diagnostics (cut-off = 0.403); HEV IgM Elisa Wantai Biopharm (cut-off = 0.263). All samples were negative except sample 33 which showed OD values of 0.421 and 0.334 in MP Diagnostic and Wantai Biopharm assays, respectively.

ISSN: 1756-0500