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Table 2 Sub-fragments of the Chinese hamster elongation factor 1 alpha downstream flanking region

From: A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments

Sub-fragment number Start* End* Length 5′-flanking RE 3′-flanking RE Clones sequenced ** Clones with the correct sequence, %
1 14545 15157 613 (Nhe I) Xba I 4 75
2 15154 15908 755 Nhe I Avr II 3 100
3 15905 16644 740 Nhe I Avr II 3 100
4 16639 17327 689 Avr II Sac I 3 100
5 17322 18019 698 Sac I Bam HI 4 100
6 18016 18794 780 Bcl I (Xho I) 5 60
  1. Nucleotide positions are numbered according to GenBank entry AY188393. Restriction sites that were used for sub-cloning the assembled fragments into the expression vectors are bracketed.
  2. *Start and end positions include the corresponding restriction endonuclease recognition sites.
  3. **Following fragment assembly, clones were re-sequenced using specific primers; no mutations were found.
  4. RE restriction endonuclease.