Sub-fragment number
|
Start*
|
End*
|
Length
|
5′-flanking RE
|
3′-flanking RE
|
Clones sequenced **
|
Clones with the correct sequence, %
|
---|
1
|
14545
|
15157
|
613
|
(Nhe I)
|
Xba I
|
4
|
75
|
2
|
15154
|
15908
|
755
|
Nhe I
|
Avr II
|
3
|
100
|
3
|
15905
|
16644
|
740
|
Nhe I
|
Avr II
|
3
|
100
|
4
|
16639
|
17327
|
689
|
Avr II
|
Sac I
|
3
|
100
|
5
|
17322
|
18019
|
698
|
Sac I
|
Bam HI
|
4
|
100
|
6
|
18016
|
18794
|
780
|
Bcl I
|
(Xho I)
|
5
|
60
|
- Nucleotide positions are numbered according to GenBank entry AY188393. Restriction sites that were used for sub-cloning the assembled fragments into the expression vectors are bracketed.
- *Start and end positions include the corresponding restriction endonuclease recognition sites.
- **Following fragment assembly, clones were re-sequenced using specific primers; no mutations were found.
- RE restriction endonuclease.