S. simulans isolates exhibit a range of patterns for FAME activity. S. simulans isolates were assayed for FAME activity/log CFU over 24 h. CFUs were generally the same among the strains with the beginning of stationary phase indicated by the arrow. Results are the average of three experiments. Vertical bars represent standard error of the observation; if no bar is apparent the standard error is smaller than the symbol. FAME activity for all S. simulans isolates was detected at 4 h (P < 0.05).