Figure 4From: Construction and use of a prokaryotic expression system for Helicobacter pylori AhpCWestern Blot showing detection of target ~52 kDa GST fusion protein by Rabbit anti H. pylori antibodies as primary antibody. Lane 1: Kaleidoscope colour marker; Lanes 2–4: Protein samples induced in E. coli BL21 cells with 0.1 mM IPTG at 0, 2 and 4 hours respectively; Lanes 6–7: Protein samples induced with 1 mM IPTG; Lanes 5 & 8: Blank controls.Back to article page