Nature of bacterial colonization influences transcription of mucin genes in mice during the first week of life

BMC Research Notes

Table 2 Primers used for qPCR

Primer name	Fwd (5´-3´)	Rev (5´-3´)	Amplicon size	Reference
Muc1	TCGTCTATTTCCTTGCCCTG	ATTACCTGCCGAAACCTCCT	185	This study
Muc2	CCCAGAAGGGACTGTGTATG	TTGTGTTCGCTCTTGGTCAG	276	Modified from [44]
Muc3	TGGTCAACTGCGAGAATGGA	TACGCTCTCCACCAGTTCCT	98	Modified from [6]
Muc4	GTCTCCCATCACGGTTCAGT	TGTCATTCCACACTCCCAGA	280	This study
Tff3	CTCTGTCACATCGGAGCAGTGT	TGAAGCACCAGGGCACATT	77	[45]
Neuroplastin	CGCTGCTCAGAACGAACCAAGAA	CTTACGGGTGGCAGTGAGTT	160	Modified from [46]
Beta-actin	GTCCACCTTCCAGCAGATGT	GAAAGGGTGTAAAACGCAGC	117	This study
Lactobacillus 16S rRNA	AGCAGTAGGGAATCTTCCA^a	CACCGCTACACATGGAG^b	341	^a[47] ^b[48]
E. coli 16S rRNA	CATGCCGCGTGTATGAAGAA	CGGGTAACGTCAATGAGCAAA	96	[49]

ISSN: 1756-0500