Biogenesis of protein bodies at mid maturation as shown in Transmission Electron Microscopy. The embryo cell ultrastructure revealed different Protein body cores. (A) Dense vesicles (dv) are shown in the cytoplasm (arrows). (B) The autophagy of a multivesicular Body (mvb) in the vacuolar compartment (vc) is shown. (C) Rough dense bodies (rdb) observed as an accumulation of precursor aggregates. (D, E) Illustrate the fusion of protein bodies through a multivesicular body (mvb) and with the association of the Rough Endoplasmic Reticulum (rER). The arrows in (D) point on the rER and in (E) on the ER derived bodies. (F, G) Remnants of the rER membrane associated with dense bodies were observed (arrows). (H) Embryo cell with different sizes dense bodies (db), which illustrate the proposed statistic model. (I) The large smooth dense body (sdb) observed in the cytoplasm, corresponds probably to its definitive size. N, Nucleus; db, dense body. The scale bar corresponds to 0,4 μm in G; 0,5 μm in B, E; 1 μm in A, D; 1,2 μm in C, I; 1,5 in F, H.