Analysis of T-DNA alleles of flavonoid biosynthesis genes in Arabidopsis ecotype Columbia

Bowerman, Peter A; Ramirez, Melissa V; Price, Michelle B; Helm, Richard F; Winkel, Brenda SJ

doi:10.1186/1756-0500-5-485

BMC Research Notes

Table 1 Summary of enzyme-encoding tt alleles described to date

From: Analysis of T-DNA alleles of flavonoid biosynthesis genes in Arabidopsis ecotype Columbia

Gene	Allele¹	Line number²	Ecotype³	Mutagen⁴	First described
chalcone synthase (CHS)at5g13930	tt4-1	85	Ler	EMS	[14, 20]
	tt4-2	2YY6	Col	EMS	[21–23]
	tt4-3	C1	Col	Carbon ions	[24]
	tt4-4	C2	Col	Carbon ions	[24]
	tt4-5	UV01	Ler	γ radiation	[25]
	tt4-6	UV25	Ler	EMS
	tt4-7	UV113	Ler	γ radiation
	tt4-8	UV118a	Ler	γ radiation
	tt4-9	38G1R	Ler	γ radiation
	tt4-10		Est-1	EMS	[26]
	tt4-11	DFW34	Ws-2	T-DNA	[27]
	tt4-12	CS429127 / GK-304D03	Col	T-DNA	[28]
	*tt4-13*	SALK_020583 ⁵	Col-0	T-DNA	[29, 30]
	tt4-14 through 21			zinc finger nucleases	[31]
chalcone isomerase (CHI)at3g55120	tt5-1	86		EMS	[14]
	*tt5-2*	CS300857/ GK-176H03	Col	T-DNA	[28, 30]; this report
	*tt5-3*	SALK_034145	Col-0	T-DNA	This report
flavanone 3-hydroxylase (F3H)at3g51240	tt6-1	87	Ler	EMS	[14, 32]
	f3h-2::En		Col	Transposon	[32]
	f3h-3::En		Col	Transposon
	f3h-4f		Col	Transposon
	f3h-5f		Col	Transposon
	tt6-2	CS427992 / GK-292E08	Col-0	T-DNA	[28]
	*tt6-3*	SALK_113904 ⁵	Col-0	T-DNA	[33]
	tt6-4	SALK_023664	Col-0	T-DNA	Leaky allele – unpublished results
flavonoid 3'-hydroxylase (F3'H)at5g07990	tt7-1	88	Ler	EMS	[14, 34]
	tt7-2		Col-7	T-DNA	[35]
	tt7-3	CS433473 / GK-349F05	Col-0	T-DNA	[28, 30]
	tt7-4	DJI11	Ws-2	T-DNA	[27]
	*tt7-5*	SALK_053394	Col-0	T-DNA	[36]
dihydroflavonol 4-reductase (DFR) at5g42800	tt3-1	84	Ler	EMS	[37]
	tt3-2	CS428258 / GK-295C10	Col-0	T-DNA	[28]
	tt3-3		Est-1	fast neutrons	[26]
		GK-212G01	Col-0	T-DNA	Some segregants have pale brown seeds, none yellow
		SALK_099848	Col-0	T-DNA	Does not have phenotype
anthocyanidin synthase (ANS/LDOX)at4g22880	tt11-1				Debeaujon and Koornneef, unpublished
	tt11-2		Ler	EMS	[38]
	tds4-1		Ws-4	T-DNA but not tagged (INRA)	[35]
	tds4-2	SALK_028793	Col-0	T-DNA	[39]
	tds4-3	CSHL GT9767	Ler	Gene trap	[39]
	tt17		Est-1	Fast neutrons	[26]
	tt18-1	AB084467	Col	Carbon ions	[40]
	tt18-2	AB084468	Col	Carbon ions
	tt18-3		Col	Carbon ions
	*tt11-11 (tds4-4)*	SALK_073183	Col-0	T-DNA	[39]; this report.
anthocyanidin reductase (ANR/BAN) at1g61720	ban-1		Ws-2	T-DNA	[41]
	ban-2	F36	En-1	unknown	[42]
	ban-3	F52	En-1	unknown	[42]
	*ban-4*	SALK_040250 ⁵	Col-0	T-DNA	This report
flavonol synthase 1 (FLS1)at5g08640	fls1-1	fls-1::En	Col	Transposon	[32, 43]
	fls-2f		Col	Transposon	[32]
	fls-3f		Col	Transposon
	fls-4d		Col	Transposon
	fls1-2	RIKEN PST16145	No-0	T-DNA	[44]
	*fls1-3*	INRA FLAG_533E06 (AJ588535/EGT283)	Ws	T-DNA	[45]
		SALK_076420	Col-0	T-DNA	Recessive embryo lethal potentially due to disruption of adjacent divergently-transcribed gene [45]
FLS2at5g63580	fls2-1	SALK_023235	Col-0	T-DNA	[45]
FLS2at5g63580	fls2-2	GK-429B10	Col-0	T-DNA	[44]
FLS3at5g63590	fls3-1	SALK_050041	Col-0	T-DNA	[44, 45]
FLS4at5g63595	fls4-1	SALK_002309	Col-0	T-DNA
FLS5at5g63600	fls5-1	CS430396 / GK-317E12	Col-0	T-DNA
FLS6at5g43935	fls6-1	SALK_003879⁵	Col-0	T-DNA

¹ Alleles in bold are described in the current study.
² GK = GABI-Kat.
³Standard ecotype abbreviations, as follows: Landsberg erecta (Ler); Columbia accession number 0 (Col-0) or accession unknown (Col), ; Enkheim (En-1); Estland (Est-1); Wassilewskija (Ws-2).
⁴ ethyl methanesulfonate (EMS).
⁵ independently-derived homozygote already available at ABRC.

Back to article page

ISSN: 1756-0500

Contact us

Submission enquiries: bmcresearchnotes@biomedcentral.com
General enquiries: ORSupport@springernature.com