Table 3 Sequencing and qPCR primers for ceramide metabolism genes
From: Characterization of genes involved in ceramide metabolism in the Pacific oyster (Crassostrea gigas)
Gene description | Elongation factor 1 α (EF1α) | Serine palmitoyltransferase (Sptlc1) | 3-ketodihydrosphingosine reductase (3KDSR) | Acid ceramidase (AC) | Glucosylceramide synthase (GlcCer) |
|---|---|---|---|---|---|
Sequencing Forward Primer | - | ATGGCGTCGACGTTCA TTCC | AGCGACGGACCGAACTTACT | TGTGATTACACGAT GTGGATACCG | AGAGGCGAACACA CGAAAGT |
Sequencing Reverse Primer | - | CTGTTCCCCAATATTTC TGAC | TGTCTTGGGTTTTGCATCCTTC | CTGCTTCTGACTTC CGGTGT | CCATATGGATAACAC TTCTCG |
Sequence Product Size (bp) | - | 1483 | 1210 | 1165 | 1080 |
qPCR Forward Primer | AAGGAAGCTGCTGA GATGGG | TTCACAGCAAGCT GAGCGAT | GCAGTGCAGTGGCTGGAAAT | TGGACTCAAGTTGG CCAGGA | TTGGCCCAAACGGG AAAGTT |
qPCR Reverse Primer | CAGCACAGTCAG CCTGTGAAGT | AAGTAGCGAGCC AACGTCAC | AGGCAGCCTTGGTGACATTG | AAGGCTGGGGGAG AGTATCG | TGTCCATGAGCGAGT CTGGT |
qPCR Product Size (bp) | 200 | 178 | 168 | 157 | 114 |