Figure 4From: Identification of suitable endogenous control genes for microRNA expression profiling of childhood medulloblastoma and human neural stem cellsQuantitative differences in miRNA expression in medulloblastoma (MB) normalising to different EC reference genes. The mean fold changes of hsa-miR-923, hsa-miR-144* and hsa-miR-21* expression in nine primary MB specimens in comparison to the mean expression of these miRNAs in CD133+ NSCs and CD133- NPCs. Relative expression was determined using the 2-ΔCq method and log2 transformed. Normalisation was carried out either to hsa-miR-425* (dark blue), RNU43 (red), hsa-miR-877 (green), RNU24 (purple) or geometric mean of hsa-miR-425* and RNU24 (light blue). The fold changes of all three miRNAs normalised against RNU43 was different from the fold changes obtained following the normalisation against hsa-miR-425*, hsa-miR-877, RNU24 and the geometric mean of hsa-miR-425* and RNU24.Back to article page