Table 2 The effect of blood volume in the Tempus tubes on RNA quality
From: Human blood RNA stabilization in samples collected and transported for a large biobank
Blood volume (ml) | Yield total RNA (ug) | RNA Integrity Number (RIN) | OD260/280 ratio | OD2602/230 ratio |
|---|---|---|---|---|
A) Adult blood samples (n = 3)* | ||||
3.0 | 7.90 ± 1.50 | 8.20 ± 0.06 | 2.19 ± 0.02 | 2.10 ± 0.23 |
2.5 | 3.38 ± 0.90 | 8.13 ± 0.27 | 2.24 ± 0.05 | 0.91 ± 0.46 |
2.0 | 2.94 ± 0.91 | 8.40 ± 0.08 | 2.12 ± 0.08 | 1.05 ± 0.53 |
1.5 | 1.38 ± 0.59 | 3.53 ± 2.53 | 2.51 ± 0.08 | 0.64 ± 0.32 |
1.0 | 0.55 ± 0.50 | 1.13 ± 0.13 | 1.19 ± 0.44 | 0.38 ± 0.38 |
0.5 | 0.07 ± 0.03 | 1.00 ± 0.00 | 0.77 ± 0.39 | 0.05 ± 0.05 |
B) Cord blood samples (n = 2) | ||||
3.3 | 79.65 ± 4.76 | 7.15 ± 0.78 | 2.15 ± 0.00 | 2.12 ± 0.00 |
3.0 | 74.60 ± 1.23 | 7.50 ± 0.65 | 2.17 ± 0.00 | 2.15 ± 0.01 |
2.0 | 44.42 ± 1.91 | 9.10 ± 0.00 | 2.18 ± 0.01 | 2.09 ± 0.01 |
1.0 | 33.92 ± 3.13 | 9.45 ± 0.04 | 1.98 ± 0.01 | 1.61 ± 0.02 |
0.5 | 4.29 ± 0.11 | 1.03 ± 0.03 | 1.25 ± 0.03 | 0.53 ± 0.01 |