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Table 2 The effect of blood volume in the Tempus tubes on RNA quality

From: Human blood RNA stabilization in samples collected and transported for a large biobank

Blood volume (ml) Yield total RNA (ug) RNA Integrity Number (RIN) OD260/280 ratio OD2602/230 ratio
A) Adult blood samples (n = 3)*
3.0 7.90 ± 1.50 8.20 ± 0.06 2.19 ± 0.02 2.10 ± 0.23
2.5 3.38 ± 0.90 8.13 ± 0.27 2.24 ± 0.05 0.91 ± 0.46
2.0 2.94 ± 0.91 8.40 ± 0.08 2.12 ± 0.08 1.05 ± 0.53
1.5 1.38 ± 0.59 3.53 ± 2.53 2.51 ± 0.08 0.64 ± 0.32
1.0 0.55 ± 0.50 1.13 ± 0.13 1.19 ± 0.44 0.38 ± 0.38
0.5 0.07 ± 0.03 1.00 ± 0.00 0.77 ± 0.39 0.05 ± 0.05
B) Cord blood samples (n = 2)
3.3 79.65 ± 4.76 7.15 ± 0.78 2.15 ± 0.00 2.12 ± 0.00
3.0 74.60 ± 1.23 7.50 ± 0.65 2.17 ± 0.00 2.15 ± 0.01
2.0 44.42 ± 1.91 9.10 ± 0.00 2.18 ± 0.01 2.09 ± 0.01
1.0 33.92 ± 3.13 9.45 ± 0.04 1.98 ± 0.01 1.61 ± 0.02
0.5 4.29 ± 0.11 1.03 ± 0.03 1.25 ± 0.03 0.53 ± 0.01
  1. Notes. *There are significant blood volume dependent reductions in the RNA yields and in the RNA qualities (RIN, OD 260/280 and OD 260/230 ratio), p < 0.05. n = number of tubes.