Table 1 Fraction yields for myoblast, myotube and AT tissue
From: A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue
Sample | Mean protein yield (μg) | Total sample protein (mg) | No. of replicates | ||
|---|---|---|---|---|---|
Nuclei | Cyto | Mito | |||
Myoblast | 1179 ± 100 (150μL) | 496 ± 25 (100μL) | 97 ± 13 (50μL) | 1.8 ± 0.1 | 5 |
Myotube | 1624 ± 107 (150μL) | 547 ± 4 (100μL) | 197 ± 15 (50μL) | 2.4 ± 0.2 | 5 |
AT tissue | 3294 ± 254 (300μL) | 1486 ± 225(300μL) | 444 ± 60 (100μL) | 5.2 ± 0.5 | 5 |