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Table 1 Fraction yields for myoblast, myotube and AT tissue

From: A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue

Sample Mean protein yield (μg) Total sample protein (mg) No. of replicates
  Nuclei Cyto Mito   
Myoblast 1179 ± 100 (150μL) 496 ± 25 (100μL) 97 ± 13 (50μL) 1.8 ± 0.1 5
Myotube 1624 ± 107 (150μL) 547 ± 4 (100μL) 197 ± 15 (50μL) 2.4 ± 0.2 5
AT tissue 3294 ± 254 (300μL) 1486 ± 225(300μL) 444 ± 60 (100μL) 5.2 ± 0.5 5
  1. Typical protein yield (μg) obtained after sample fractionation using the indicated volume of lysis buffer for myoblasts, myotubes (both cultured in 75 cm2 flask, ≈2×106 cells) and AT muscle (all n = 5, mean ± SEM). The protein yields (determined by BCA assay as total volume of a fraction × protein concentration in the fraction) are representative and dependent upon the volume of buffer utilized to resuspend each fraction.