Figure 1
From: DICER1 RNase IIIb domain mutations are infrequent in testicular germ cell tumours
HRM analysis detection of previously identified mutations within the DICER1 RNase IIIb domain. Nucleotide and amino acid numbering are based on DICER1 reference sequence [NCBI:NM_177438]. 1A. Aberrant HRM curves resulting from four different heterozygous mutations, affecting amino acids 1705 and 1709 of DICER1. #1 = c.5113G>A (p.E1705K); #2 = c.5125G>A (p.D1709N); #3 = c.5126A>G(p.D1709G); #4 = c.5127T>A (p.D1709E); Ref = reference sequence. 1B. Aberrant HRM curves resulting from two different heterozygous mutations, affecting amino acids 1810 and 1813 of DICER1. #1 = c.5428G>T (p.D1810Y); #2 = c.5437G>C (E1813Q); Ref = reference sequence.