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Figure 3 | BMC Research Notes

Figure 3

From: Bacterial and fungal chitinase chiJ orthologs evolve under different selective constraints following horizontal gene transfer

Figure 3

Reverse conservation analysis of ChiJ orthologs. Amino acid conservation was estimated using Rate4Site, based on a Clustal X alignment of ChiJ Streptomyces orthologs, and plotted as W mean scores in arbitrary units (solid line). Amino acid conservation was estimated in a similar way for fungal orthologs in a previous work [10], and included in the figure (dashed line). Horizontal line indicates a 0.5 standard deviation cut-off. The x-axis represent residue position, asterisks (*) indicate positions of predicted catalytic residues, diamonds () indicate predicted substrate-interacting residues, boxed P indicate residues under strong (Bayes factor ≥ 50) positive selection, P indicate residues under weak (Bayes factor 10–49) positive selection, boxed C interconnected by horizontal lines indicate co-evolving residue groups and vertical dashed lines indicate identical residues. The position of highly variable regions successfully visualised by homology modelling are indicated (I through VII). Magnifications illustrate residue S score distribution of the selected region. The position of a highly variable region in the fungal orthologs is indicated by A.

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