Table 1 Primer sequences and accession numbers used for real-time PCR
Target gene | Primer sequence | Accession number |
|---|---|---|
TLR2 | F: 5’- ATCCTGCTGGAGCCCATTCAGAG -3’ | [GenBank: NM_204278.1/NM_001161650] |
R: 5’- TTGCTCTTCATCAGGAGGCCACTC -3’ | ||
TLR3 | F: 5’- TCAGTACATTTGTAACACCCCGCC -3’ | [GenBank: DQ780341] |
R: 5’- GGCGTCATAATCAAACACTCC -3’ | ||
TLR4 | F: 5’- TGCCATCCCAACCCAACCACAG -3’ | [GenBank: AY064697.1] |
R: 5’- ACACCCACTGAGCAGCACCAA -3’ | ||
TLR5 | F: 5’- TTCTTGCAACCTCACAGGTGTTCC -3’ | [GenBank: NM_001024586.1] |
R: 5’- CAGGTCCAAGACACGAAGATT -3’ | ||
TLR7 | F: 5’- TTCTGGCCACAGATGTGACC -3’ | [GenBank: NM_001011688] |
R: 5’- CCTTCAACTTGGCAGTGCAG -3’ | ||
TLR21 | F: 5’- CCTGCGCAAGTGTCCGCTCA -3’ | [GenBank: AJ720600.1] |
R: 5’- GCCCCAGGTCCAGGAAGCAG -3’ | ||
IFN-γ | F: 5’- ACACTGACAAGTCAAAGCCGCACA-3’ | [GenBank: X99774] |
R: 5’-AGTCGTTCATCGGGAGCTTGGC-3’ | ||
IL-4 | F: 5’-TGTGCCCACGCTGTGCTTACA-3’ | [GenBank: AJ621249.1] |
R: 5’- CTTGTGGCAGTGCTGGCTCTCC-3’ | ||
IL-10 | F: 5’- AGCAGATCAAGGAGACGTTC −3’ | [GenBank: AJ621614] |
R: 5’- ATCAGCAGGTACTCCTCGAT −3 | ||
IL-13 | F: 5’- ACTTGTCCAAGCTGAAGCTGTC -3’ | [GenBank: AJ621250] |
R: 5’- TCTTGCAGTCGGTCATGTTGTC -3’ | ||
IL-17 | F: 5’- CACTGCTGTTGGTGTTGCT -3’ | [GenBank: AJ493595] |
R: 5’- TCAGCAACCAAGCGGGGG -3’ | ||
β-Actin | F: 5’-CAACACAGTGCTGTCTGGTGGTA-3’ | [GenBank: X00182] |
R: 5’-ATCGTACTCCTGCTTGCTGATCC-3’ |