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Table 3 Comparison of the reliability of estimating A-to-I RNA editing of the Dα6 subunit using the peak height ratio method between Dα6IR2 and Dα6285F primers at different rates of expected editing

From: Antisense sequencing improves the accuracy and precision of A-to-I editing measurements using the peak height ratio method

  Editing Site
Seq. Primer 398 400 415 416
Dα6IR2 0.98 ± 0.03
(1.00)
1.00 ± 0.03
(1.00)
0.97 ± 0.05
(1.00)
0.97 ± 0.05
(1.00)
Dα6285F 0.94 ± 0.10
(0.99)
0.88 ± 0.10*
(0.99)
0.80 ± 0.07*
(0.99)
0.85 ± 0.04*
(1.00)
  1. Values are the slopes and associated confidence intervals of editing estimate vs. expected values. Numbers in parentheses indicate the r2 value. Slopes with * indicate values that are significantly different than 1.0 (i.e., 95% CI does not include 1.0)