Figure 2
Top – capture and reporter aptamer sandwich matrix used to screen for the best fluorescent aptamer-MB combination. Bottom – bar graph showing average peak heights at 658 nm (using TYE 665 fluorophore) for three separate measurements. Error bars represent the range of the three peak heights. Excitation was at 645 nm with 5 nm slits and emissions were scanned from 655 to 720 nm with a photomultiplier (PMT) setting of 900 V.