Figure 3

Schematic of the refined combination 20 CCHF prototype assay showing the 146 poly-adenine spacer tail with a terminal 5’-biotin for coupling to streptavidin (SAv)-coated MBs and the cut sites where the capture and reporter aptamers were truncated during synthesis to prevent partial hybridization in the absence of the virus target, thereby lowering background fluorescence. The secondary structures of the capture and reporter aptamers were used to determine where the primer regions could hybridize and were truncated.