Table 1 Identification of the best pairs of genes for different treatments, species, and tissues
From: Validation of reference genes from Eucalyptus spp. under different stress conditions
A | |||||||
|---|---|---|---|---|---|---|---|
Species | Software | CTRL | DRT | REC | CTRL+DRT+REC Apex | CTRL+DRT+REC Base | All treatments |
E. globulus | GeNorm | A-Tub/UBQ | A-Tub/H2B | IDH/SAND | UBQ/H2B | H2B/A-Tub | H2B/UBQ |
NormFinder | IDH/ACT | ACT/A-Tub | A-Tub/IDH | IDH/A-Tub | A-Tub/ACT | A-Tub/ACT | |
E. urograndis | GeNorm | SAND/A-Tub | H2B/UBQ | A-Tub/SAND | UBQ/A-Tub | IDH/ACT | IDH/ACT |
NormFinder | ACT/SAND | UBQ/H2B | SAND/A-Tub | ACT/SAND | A-Tub/SAND | ACT/SAND | |
E. uroglobulus | GeNorm | H2B/UBQ | EF1/IDH | IDH/ACT | SAND/IDH | IDH/18S | A-Tub/ACT |
NormFinder | A-Tub/EF1 | SAND/ACT | 18S/H2B | H2B/A-Tub | A-Tub/ACT | A-Tub/H2B | |
All species | GeNorm | UBQ/H2B | IDH/SAND | IDH/ACT | IDH/ACT | IDH/ACT | IDH/ACT |
NormFinder | IDH/SAND | ACT/IDH | SAND/IDH | SAND/ACT | SAND/IDH | SAND/IDH | |
B | |||||||
Species | Software | CTRL | COLD | CTRL+COLD Apex | CTRL+COLD Base | All treatments | |
E. globulus | GeNorm | A-Tub/UBQ | EF1/IDH | EF1/IDH | SAND/EF1 | EF1/IDH | |
NormFinder | ACT/UBQ | SAND/UBQ | ACT/A-Tub | ACT/SAND | ACT/A-Tub | ||
E. urograndis | GeNorm | IDH/UBQ | A-Tub/UBQ | UBQ/A-Tub | UBQ/18S | A-Tub/UBQ | |
NormFinder | IDH/UBQ | SAND/UBQ | SAND/IDH | ACT/IDH | IDH/SAND | ||
E. uroglobulus | GeNorm | IDH/ACT | UBQ/SAND | SAND/UBQ | B-tub/SAND | UBQ/SAND | |
NormFinder | A-Tub/H2B | ACT/IDH | H2B/UBQ | A-Tub/ACT | H2B/A-Tub | ||
All species | GeNorm | SAND/UBQ | ACT/SAND | UBQ/IDH | IDH/SAND | IDH/SAND | |
NormFinder | A-Tub/EF1 | IDH/ACT | H2B/UBQ | IDH/SAND | IDH/SAND | ||
C | |||||||
Species | Software | DRT+COLD Apex | DRT+COLD Base | All treatments | |||
E. globulus | GeNorm | IDH/A-Tub | IDH/SAND | IDH/ACT | |||
NormFinder | UBQ/IDH | UBQ/A-Tub | UBQ/A-Tub | ||||
E. urograndis | GeNorm | H2B/UBQ | SAND/A-Tub | IDH/ACT | |||
NormFinder | ACT/A-Tub | SAND/A-Tub | ACT/IDH | ||||
E. uroglobulus | GeNorm | IDH/ACT | EF1/IDH | IDH/ACT | |||
NormFinder | H2B/A-Tub | A-Tub/ACT | A-Tub/H2B | ||||
All species | GeNorm | ACT/IDH | IDH/SAND | IDH/ACT | |||
NormFinder | ACT/IDH | SAND/IDH | IDH/ACT | ||||
D | |||||||
Species | Software | in vitro (dark and light) | Greenhouse (dark and night) | All treatments | |||
E. globulus | GeNorm | H2B/IDH | EF1/ACT | UBQ/IDH | |||
NormFinder | UBQ/ACT | IDH/EF1 | ACT/IDH | ||||