Table 1 Primer and probe sequences for qPCR used in the present study
Gene | GenBank # | Primer forward 5'-3' | Primer reverse 5'-3' | Hydrolysis probe 5'-3' | Amplicon size (bp)* | Reference |
|---|---|---|---|---|---|---|
EF1A A | AF321836 | CCCCTCCAGGACGTTTACAAA | CACACGGCCCACAGGTACA | 6-FAM-ATCGGTGGTATTGGAAC-MGB | 57 | [34] |
HSP70 | BG933934 | CCCCTGTCCCTGGGTATTG | CACCAGGCTGGTTGTCTGAGT | 6-FAM-CGCTGGAGGTGTCATG-MGB | 121 | [52] |
GR | BG934480 | CCAGTGATGGCTTTTTTGAACTT | CCGGCCCCCACTATGAC | 6-FAM-AGCCTTCCTAAGCGCAG-MGB | 61 | [52] |
Cu/Zn SOD | BG936553 | CCACGTCCATGCCTTTGG | TCAGCTGCTGCAGTCACGTT | 6-FAM-ACAACACCAACGGCT-MGB | 140 | [55] |
Mn SOD | DY718412 | GTTTCTCTCCAGCCTGCTCTAAG | CCGCTCTCCTTGTCGAAGC | 6-FAM-CACATCAACCACACCATCTTCTGGACAAAC-TAMRA | 209 | [53] |
GP | BG934453 | GATTCGTTCCAAACTTCCTGCTA | GCTCCCAGAACAGCCTGTTG | 6-FAM-TGAATGGAGACACAGAAC-MGB | 140 | [55] |
IGF-1 | M81904 | GTGTGCGGAGAGAGAGGCTTT | TGTGACCGCCGTGAACTG | 6-FAM-TTTCAGTAAACCAACGGGCTATGG-TAMRA | 68 | [54] |
IGF-1R | AY049954 | TGAAGAGCCACCTGAGGTCACT | TCAGAGGTGGGAGGTTGAGACT | 6-FAM-CGGGCTAAAGACCCGTCCCAGTCC-TAMRA | 72 | [54] |