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Table 3 The effect of treatment, type and their interaction on fish weight and gene expressions

From: Effects of environmental stress on mRNA expression levels of seven genes related to oxidative stress and growth in Atlantic salmon Salmo salar L. of farmed, hybrid and wild origin

Salmo salar L.

n

Effects

Fixed

Random

DFn

DFd

Sum Sq

F

P

Weight [log]

180

Treatment

Treatment:Tank

1

28

0.62

23.19

<0.0001***

  

Type

 

2

27

6.06

113.11

<0.0001***

Gene

n

  

DFn

DFd

 

F

P

EF1A A

177

Type

Treatment:Tank

2

26

0.0002

1.17

0.3

HSP70

173

Treatment

Treatment:Tank

1

26

0.0017

0.72

0.3

GR

173

Type

Treatment:Tank

2

25

0.08

7.92

0.001***

Cu/Zn SOD

166

Type

Treatment:Tank

2

24

0.06

3.49

0.04*

Mn SOD

170

Type

Weight [log]

2

24

0.03

5.42

0.01**

GP

172

Type

Weight [log]

2

24

0.07

6.79

0.003**

IGF-1

168

Treatment

Weight [log]

1

25

0.09

13.57

<0.001***

  

Type

 

2

24

0.15

10.97

<0.001***

IGF-1R

173

Type

Treatment:Tank

2

23

0.01

1.37

0.24

  1. Summary of the best linear mixed effect models testing for difference in fish weight, and mRNA levels (raw Cq values of EF1AA, ΔΔCq values of target genes) between treatments and genetic origins (type). Including the interaction between treatment and type did not improve the fit of any of the models, thus all groups were affected by treatment in the same matter. The random effects were included by forward selection. Treatment:Tank; tank nested within treatment, Weight [log]; log transformed weight measurement of the fish. For more information on model selection based upon AIC values, see Additional file 1. The statistical significance is marked with asterisks, where * = P ≤ 0.05, ** = P ≤ 0.01 and *** = P ≤ 0.001.