Table 1 Primer sequences and amplicon information for the quadruplex HRM PCR assay
From: Rapid multiplex high resolution melting method to analyze inflammatory related SNPs in preterm birth
SNP reference number | Gene | Chromosome | Primer sequence (5'-> 3') | PCR product size (bp) | Primer concentration in multiplex PCR (μM) | |
|---|---|---|---|---|---|---|
rs4986790 | TLR4 | 9 | F | ATTTGACCATTGAAGAATTCCG | 157 | 2.46 |
| Â | Â | Â | R | TGTTGCCATCCGAAATTATAAG | Â | Â |
rs1800795 | IL6 | 7 | F | GCCTCAATGACGACCTAAGC | 105 | 0.46 |
| Â | Â | Â | R | GGGGCTGATTGGAAACCTTA | Â | Â |
rs16944 | IL1β | 2 | F | CTTGGGTGCTGTTCTCTGCCTC | 126 | 0.26 |
| Â | Â | Â | R | CAACTCCGTCAGGAGCCTGAAC | Â | Â |
rs375947 | IL12RB | 19 | F | CTGCCATTCAATGCAATACG | 241 | 0.9 |
| Â | Â | Â | R | CCCTGTAGGGTCAGGGGTAT | Â | Â |