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Table 1 Phenotyping panel used for cells subsets detection

From: Haematological parameters, natural regulatory CD4 + CD25 + FOXP3+ T cells and γδ T cells among two sympatric ethnic groups having different susceptibility to malaria in Burkina Faso

Phenotyping Panel

Antibodies - fluorochromes

Ex-vivo staining

 

T cells

CD3-PE/CD4-PC5/CD25-FITC

γδ T cells

CD3-PE/Panγδ-PC5/Vδ2-FITC

CD127 T cells

CD127-PE/CD4-PC5/CD25-FITC

FoxP3 T cells

FoxP3-PE/CD4-PC5/CD25-FITC

Stimulated cells

 

IL10 secreting T cells

IL10-PE/CD4-PC5/CD25-FITC

IFNγ secreting γδ T cells

IFNγ-PE/Panγδ-PC5/Vδ2-FITC

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BMC Research Notes

ISSN: 1756-0500

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