Effect of the isolation procedure on the surface expression of mCD14 on monocytes. Whole blood monocytes or isolated PBMC was stained with CD14 mAb. Blood was anticoagulated with HEPARIN. The percentage of monocytes expressing mCD14 (4A) and the mean channel fluorescence intensity (MFI) (4B) was measured. 30,000 cells were gated and analysed for each event. Results for each treatment are the mean from 5 cows.