Iron detection in the rat maxillary third molar. Sagittal section of 4-week old rat maxillary molars were stained with DAB enhanced Perls’ blue for iron and counterstained with fast red. Panel A: Section of three molars observed under stereoscope with 16x magnification. Panel B: The third molar shown with 40x magnification. Panels C1 - C5: Regions 1 - 5 as identified in panel B are shown with 100x magnification. Panels D1 - D5: Ameloblasts and papillary layer cells, corresponding again to regions 1 - 5 as identified in panel B, are shown with 630x magnification. Ameloblasts (am) and papillary layer cells (pl) are labeled in panel D1. The arrows in D2 and D5 show iron deposits in papillary layer cells. Scale bars: A and B, 1 mm; C1-C5 and D1-D5, 10 μm.