Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

Table 3 The sensitivity and specificity of CD4 T cell counting by the Auto40 to identify patients having less than (or more than) 200 CD4 T cells/μl, 350 CD4 T cells/μl, 750 CD4 T cells/μl and 25%CD4+, calculated on the 258 available CD4 T cell count measurements

* 200 CD4 T cells/μl: Threshold of immune-restoration under antiretroviral treatment and the threshold for therapeutic initiation according to the 2006-revised WHO recommendations [30];
** 350 CD4 T cells/μl: New WHO threshold for antiretroviral treatment initiation in adults and children aged more than 5 years [3];
*** 750 CD4 T cells/μl and 25%CD4+: WHO thresholds for antiretroviral treatment initiation in children aged between 24 and 59 months [2];
^$A 10% bilateral range (i.e., counts between 190 and 210 CD4 T cells/μl for the threshold at 200 CD4 T cells/μl; counts between 332 and 367 CD4 T cells/μl for the threshold at 350 CD4 T cells/μl; counts between 712 and 787 CD4 T cells/μl for the threshold at 750 CD4 T cells/μl; and counts 23.7 and 26.2%CD4+ for the threshold at 25%CD4+) were considered similar.

ISSN: 1756-0500