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Table 3 The sensitivity and specificity of CD4 T cell counting by the Auto40 to identify patients having less than (or more than) 200 CD4 T cells/μl, 350 CD4 T cells/μl, 750 CD4 T cells/μl and 25%CD4+, calculated on the 258 available CD4 T cell count measurements

From: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

   Sensitivity Specificity Cohen’s κ coefficient$
  200 CD4 T cells/μl* 89% 99% 0.97
Thresholds 350 CD4 T cells/μl** 94% 98% 0.98
  750 CD4 T cells/μl*** 99% 96% 0.97
  25%CD4 94% 98% 0.96
  1. * 200 CD4 T cells/μl: Threshold of immune-restoration under antiretroviral treatment and the threshold for therapeutic initiation according to the 2006-revised WHO recommendations [30];
  2. ** 350 CD4 T cells/μl: New WHO threshold for antiretroviral treatment initiation in adults and children aged more than 5 years [3];
  3. *** 750 CD4 T cells/μl and 25%CD4+: WHO thresholds for antiretroviral treatment initiation in children aged between 24 and 59 months [2];
  4. $A 10% bilateral range (i.e., counts between 190 and 210 CD4 T cells/μl for the threshold at 200 CD4 T cells/μl; counts between 332 and 367 CD4 T cells/μl for the threshold at 350 CD4 T cells/μl; counts between 712 and 787 CD4 T cells/μl for the threshold at 750 CD4 T cells/μl; and counts 23.7 and 26.2%CD4+ for the threshold at 25%CD4+) were considered similar.