Amplification of the hypervariable regions of kDNA minicircle molecules of three T . cruzi populations. The PCR assays were performed in triplicate by three independent reactions for each T. cruzi population (CL Brener clone, Col1.7G2 clone or JG strain), as indicated in the figure. NC: negative control (without parasite DNA). MM: molecular size marker (1 Kb Plus DNA ladder - Invitrogen®).