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Table 1 Sequence of primers designed to analyze three T . cruzi populations by LSSP-PCR

From: LSSP-PCR of Trypanosoma cruzi: how the single primer sequence affects the kDNA signature

Primer code Original name Sequence (5′ → 3′) Size (bp) Reference
   1                  26   
C S35 AAATAATGTACGGGKGAGATGCATGA 26 [20], [22], [34], [40]
A S35 ---ATAATGTACGGGKGAGATGC---- 20 [42]
B S35 AAATAATGTACGGG-GAGATGCATGA 25 [23], [43]–[45]
D S35 AAATAATGTACGGGG GAGATGCATGA 26 [7], [21]
E S35G -----ATGTACGGG-GAGATGCATGA 20 [17], [35], [46]
F S35G* -----ATGTACGGGG GAGATGCATGA 21 [19]
G S35G AAATAATGTACGGGG GAGATG----- 21 [41], [47]
H S35G 5 AAATAATGTACGGGG GAGAT------ 20 Not published
  1. *Indicates that this primer was originally described as fluorescein labeled but here the fluorescent marker was removed. All changes relative to primer S35 described by Sturm et al. (primer C) are highlighted.