LSSP-PCR of Trypanosoma cruzi: how the single primer sequence affects the kDNA signature

BMC Research Notes

Table 1 Sequence of primers designed to analyze three T . cruzi populations by LSSP-PCR

Primer code	Original name	Sequence (5′ → 3′)	Size (bp)	Reference
		_{1 26}
C	S35	AAATAATGTACGGGKGAGATGCATGA	26	[20], [22], [34], [40]
A	S35	---ATAATGTACGGGKGAGATGC----	20	[42]
B	S35	AAATAATGTACGGG-GAGATGCATGA	25	[23], [43]–[45]
D	S35	AAATAATGTACGGGG GAGATGCATGA	26	[7], [21]
E	S35G	-----ATGTACGGG-GAGATGCATGA	20	[17], [35], [46]
F	S35G*	-----ATGTACGGGG GAGATGCATGA	21	[19]
G	S35G	AAATAATGTACGGGG GAGATG-----	21	[41], [47]
H	S35G 5	AAATAATGTACGGGG GAGAT------	20	Not published

*Indicates that this primer was originally described as fluorescein labeled but here the fluorescent marker was removed. All changes relative to primer S35 described by Sturm et al. (primer C) are highlighted.

ISSN: 1756-0500