Figure 4
From: PCR on yeast colonies: an improved method for glyco-engineered Saccharomyces cerevisiae
MNN5 gene amplification efficiency on modified strain colonies picked from Petri dishes or micro-plate cultures. A) 3 independent assays carried out on 47 colonies for each condition. Dark grey: Petri dishes cultures, light grey: micro-plate cultures. B) Average of the 3 assays presented in A (standard deviation =22.07). C) PCR product visualised on a 1% agarose gel stained with SYBR safe. Left panel (1): Petri dishes, right panel (2): micro-plate cultures, ML: Molecular ladder. All amplifications were carried out using Platinium Taq.