Distribution of CCR5-Delta32, CCR5 promoter 59029 A/G, CCR2-64I and SDF1-3’A genetic polymorphisms in HIV-1 infected and uninfected patients in the West Region of Cameroon

Table 1 Primers, amplicon size, enzymes, expected fragments size after digestion and references used for genotyping

Genes	Primers sequence (5’-3’)	Size of the amplicon (bp)	Enzyme used for the RFLP	Expected fragments size (bp)	Reference
CCR5-Δ32	CTTCATCATCCTCCTGACAATCG	262 (wt)	None	262 or 230	[33]Kristiansen et al., 2001
CCR5-Δ32	GACCAGCCCCAAGTTGACTATC	230 (mut)			[33]Kristiansen et al., 2001
CCR5 promoter A/G	TGGGGTGGGATAGGGGATAC	498	Bsp 1286I	453 + 45	[33]Kristiansen et al., 2001
CCR5 promoter A/G	TGTATTGAAGGCGAAAAGAATCAG				[33]Kristiansen et al., 2001
CCR2-64I	GGATTGAACAAGGACGCATTTCCCC	380	Fok I	215 +165	[34]Magierowska et al., 1999
CCR2-64I	TTGCACATTGCATTCCCAAAGACCC				[34]Magierowska et al., 1999
SDF1-3’A	CAGTCAACCTGGGCAAAGCC	302	Msp I	202+ 100	[33]Kristiansen et al., 2001
	AGCTTTGGTCCTGAGAGTCC

ISSN: 1756-0500