Table 7 Details of primers used for the cDNA amplification of six candidate reference genes
Name | Accession number | Forward primer 5′-3′ | Reverse primer 5′-3′ | Length (bp) | Tm(°C) |
|---|---|---|---|---|---|
18S rRNA | X67238 | CGCGCAAATTACCCAATCCTGACA | TCCCGAAGGCCAACGTAAATAGGA | 416 | 60 |
APRT | CK270447 | TGGCGCCTCATGATCCGATTCTTA | ACTCCAACACGCTCAAGAAGCCTA | 454 | 60 |
GAPDH | U17005 | AACCGGTGTCTTCACTGACAAGGA | GCTTGACCTGCTGTCACCAACAAA | 586 | 60 |
Cyclophilin | AF126551 | AGGGTTCATGTGTCAAGGAGGTGA | TCCAACAGCCTCGGCCTTCTTAAT | 265 | 60 |
Actin | X55749 | TGTGTTGGACTCTGGTGATGGTGT | AATAGGACCTCAGGGCAACGGAAT | 333 | 60 |
RuBP | FS083182 | GCAGGTGTGGCCACCAATTAACAA | TGCACTCTCCGACCTCATTCAACA | 269 | 60 |